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Title of article INVESTIGATING ANTIBIOTIC ACTIVITY OF THE GENUS BACILLUS STRAINS AND PROPERTIES OF THEIR BACTERIOCINS IN ORDER TO DEVELOP NEXT-GENERATION PHARMACEUTICALS
Authors

Zimina M.I. , Kemerovo Institute of Food Science and Technology (University) , Stroiteley blvd. 47, Kemerovo, 650056 Russian Federation , mariia.zimina@list.ru

Sukhih S.A. , Kemerovo Institute of Food Science and Technology (University) , Stroiteley blvd. 47, Kemerovo, 650056 Russian Federation

Babich O.O. , Kemerovo State University , Krasnaya Str. 6, Kemerovo, 650043 Russian Federation

Noskova S.Yu. , Kemerovo Institute of Food Science and Technology (University) , Stroiteley blvd. 47, Kemerovo, 650056 Russian Federation

Abrashina A.A. , Kemerovo Institute of Food Science and Technology (University) , Stroiteley blvd. 47, Kemerovo, 650056 Russian Federation

Prosekov A.Yu. , Kemerovo State University , Krasnaya Str. 6, Kemerovo, 650043 Russian Federation

Section: BIOTECHNOLOGY
Year 2016 Issue 2 DOI 10.21179/2308-4057-2016-2-92-100
Annotation In recent years, we have witnessed a considerable growth in number of strains resistant to antibiotics. Therefore, research on new antimicrobial components that might be used for development of new-generation drugs is currently very important. We have studied antibiotic activity of Bacillus safensis, Bacillus endopheticus, Bacillus subtilis strains, isolated their bacteriocins, and evaluated their properties. The study was carried out in the scientific research institute for biotechnology, Kemerovo Institute of Food Science and Technology, in the city of Kemerovo. Strains of microorganisms were isolated from vegetables grown in Krasnodar region, namely, samples of Manas onions, Big Beef tomatoes, and Capia bell peppers. Antibiotic activity of the strains was evaluated in liquid nutrient medium. All test strains demonstrated some level of antimicrobial activity which varied from 18 to 91%. We established minimum inhibitory concentrations for the isolated strains based on measured optical density; MIC for Bacillus safensis was 1.5*106 CFU/сm3, for Bacillus endopheticus, 1.5*106 CFU/сm3, for Bacillus subtilis, 1.5*106 CFU/сm3. We then isolated respective bacteriocins and purified them by HPLC method. During disk diffusion tests, bacteriocin preparations proved active against Micrococcus luteus strain. Molecular weight was determined by PAGE electrophoresis. Molecular weight of bacteriocins varied from 3.6 through 4.21 kDа. Isolated bacteriocins were proved to belong to the lantibiotics class.
Keywords antibacterial activity, Bacillus strains, bacteriocins, pathogenic strains
Artice information Received August 17, 2016
Accepted October 24, 2016
Available online December 31, 2016
Imprint article Zimina M.I., Sukhih S.A., Babich O.O., Noskova S.Yu., Abrashina A.A., and Prosekov A.Yu. Investigating antibiotic activity of the genus Bacillus strains and properties of their bacteriocins in order to develop next-generation pharmaceuticals. Food and Raw Materials, 2016, vol. 4, no. 2, pp. 92-100. doi: 10.21179/2308-4057-2016-2-92-100
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