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Patrakova I.S. , Kemerovo Institute of Food Science and Technology (University) , bul'v. Stroiteley 47, Kemerovo, 650056 Russia ,

Gurinovich G.V. , Kemerovo Institute of Food Science and Technology (University) , bul'v. Stroiteley 47, Kemerovo, 650056 Russia

Year 2015 Issue 1 DOI 10.12737/11235
Annotation Oxidation of lipids and myoglobin in raw meat are interrelated processes that affect the overall meat quality. The intensity of oxidation processes in meat raw material is regulated by its own antioxidant system (catalase, peroxidase, glutathione, etc.), the activity of which should be considered in the development of new technological solutions. Oxidation of lipids and myoglobin, directly affect the quality and safety of meat products, and reducing of the intensity of these processes contributes to the life time of raw meat, as well as that of finished products. The paper presents the study results of the salt curing mixture, including combination with yeast extract, affecting on the activity of the antioxidant system of the main types of raw meat - pork and beef. The basic systems, minced pork and beef being subjected to salting with curing salt (sodium chloride) and curing mixture consisting of 70% sodium chloride and 30% of composition KCl + CaCl at the ratio of 1:1 are investigated. The influence of curing mixture on the intensity of oxidation of lipids and myoglobin of raw meat of different species is stated. It is found, that reducing the amount of sodium chloride in curing composition of the mixture reduces oxidative changes of heme pigments and meat lipids. Introduction of yeast extract into raw meat, in the amount of 2% enhances the inhibitory effect on oxidation in raw minced meat.
Keywords Antioxidant system, antioxidant enzymes, catalase, peroxidase, meat, myoglobin, metmyoglobin, lipids, oxidation, yeast extract
Artice information Received February 17, 2015
Accepted March 5, 2015
Available online June 30, 2015
Imprint article Patrakova I.S., and Gurinovich G.V. The study of factors affecting the activity of meat antioxidant system. Food and Raw Materials, 2015, vol. 3, no. 1, pp. 33-40
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  1. Min, B., Cordray, J.C., and Ahn, D.U., Effect of NaCl, Myoglobin, Fe(II), and Fe(III) on lipid oxidation of raw and cooked chicken breast and beef loin, J. Agric. Food Chem., 2010, vol. 58, рp. 600–605.
  2. Lynch, M.P., Faustman, C., Silbart, L.K., Rood, D., and Furr, H.C., Detection of lipid-derived aldehydes and aldehyde: protein adducts in vitro and in beef, J. Food Sci., 2001, vol. 66, pp. 1093–1099.
  3. Morrissey, P.A., Sheehy, P.A., Galvin, K., Kerry, J.P., and Buckley, D.J., Lipid stability in meat and meat products, Meat Sci., 1998, vol. 49, pp. 73–86.
  4. Min, B., and Ahn, D.U., Mechanism of lipid peroxidation in meat and meat products – A Review, Food Sci. Biotechnol., 2005, vol. 14, no. 1, pp. 152–163.
  5. Kanner, J., Oxidative processes in meat and meat products: Quality implications, Meat Sci., 1994, vol. 36, pp. 169–189.
  6. Medvedev, Ya.V., and Shleikin, A.G., Gem-zavisimoe perekisnoe okislenie v mjase pri holodil'nom hranenii (Haem-dependent peroxidation in meat during cold storage), Vestnik Mezhdunarodnoi Akademii Kholoda (Journal of IAR), 2013, no. 2, pp. 57–61.
  7. Gheisari, Hamid R., M?ller, Jens K.S. Adamsen, Ch., Skibsted, Leif H., Sodium chloride or heme protein induced lipid oxidation in raw, minced chicken meat and beef, Czech J. Food Sci., 2010, vol. 28, no. 5, pp. 364–375.
  8. Manat Chaijan, Review: Lipid and myoglobin oxidations in muscle foods, Songklanakarin J. Sci. Technol., 2008, vol. 30 (1), pp. 47–53.
  9. Johns, A.M., Birkinshaw, L.H., and Ledward, D.A., Catalysts of lipid oxidation in meat products, Meat Sci., 1989, vol. 25, pp. 209–220.
  10. Kanner, J., Hazan, B., and Doll, L. Catalytic “free” iron ions in muscle foods, J. Agric. Food Chem, 1988, vol. 36, pp. 412–415.
  11. Ahn, D.U., and Kim, S.M., Prooxidant effects of ferrous iron, hemoglobin, and ferritin in oil emulsion and cooked-meat homogenates are different from those in raw meat homogenates, Poultry Science, 1988, vol. 77, pp. 348–355.
  12. Rao, S.I., Wilks, A., Hamberg, M., Ortiz de Montellano, P.R., The lipoxygenase activity of myoglobin. Oxidation of linoleic acid by the ferryl oxygen rather than protein radical, J. Biol. Chem, 1994, 269, pp. 7210–7216.
  13. Aeby, H., Catalase in vitro, Methods Enzymol, 1984, vol. 105, pp. 121–126.
  14. Baron, C.P., and Andersen, H.J., Myoglobin-induced lipid oxidation, Journal of Agricultural and Food Chemistry, 2002, vol. 50, pp. 3887–3897.
  15. Metelica, D.I., and Karaseva, E.I., Iniciirovanie ingibirovanie svobodnoradikal'nyh processov v bio-himicheskih peroksidaznyh sistemah (Initiating of free radical processes in biochemical peroxidase systems), Prikladnaya Biokhimiya i Mikrobiologiya (Applied Biochemistry and Microbiology), 2007, vol. 43, no. 5, pp. 537–564.
  16. Gheisari, H., and Motamedi, H., Chloride salt type/ionic strength and refrigeration effects on antioxidant enzymes and lipid oxidation in cattle, camel and chicken meat, Meat Sci., 2010, vol. 86(2), рp. 377–383.
  17. Gheisari, Hamid Reza, Correlation between acid, TBA, peroxide and iodine values, catalase and glutathione peroxidase activities of chicken, cattle and camel meat during refrigerated storage, Veterinary World, 2011, vol. 4(4), pp.153–157.
  18. Gostjuhina, O.L., Golovina, I.V., and Vahtina, T.B., Antioksidantnyj kompleks kombaly-kalkana Psetta (Scophthalmus) maxima maeotica (L., 1758) kak indikator fiziologicheskogo sostojanija organizma: tkanevye osobennosti (The antioxidant complex of the turbot Psetta (Scophthalmus) maxima maeotica (L., 1758) as indicator of phys-iological state of organism: tissue peculiarities), Morskoj ehkologicheskij zhurnal (Marine ecological journal), 2010, no. 3, pp. 15–22.
  19. McLean, R.M., Mann, J.I., and Hoek, J., World Salt Awareness Week: more action needed in New Zealand, The New Zealand Medical Journal, 2011, vol. 124, no. 1332, pр. 68–77.
  20. Hernandez, P., Lopez, A., Marco, M., and Blasco, A., Influence of muscle type, refrigeration storage and genetic line on antioxidant enzyme activity in rabbit meat, World Rabbit Science, 2005, vol. 10, no. 4, pp. 141–146.
  21. Luciano, G., Monahan, F. J., Vasta, V., Pennisi P., Bella M. and Priolo, A., Lipid and color stability of meat from lambs fed fresh herbage or concentrate, Meat Sci., 2009, vol. 82 (2).
  22. Kenija, M.V., Gus'kov, E.P., and Lukash, A.I., Rol' nizkomolekuljarnyh antioksidantov pri okislitel'nom stresse (The Role of low-molecular antioxidants in oxidizing stress), Uspehi sovrem. biologii (Successes of Modern Biology), 1993, vol. 4, pp. 456–470.
  23. Kanner, J., Harel, S., and Jaffe, R., Lipid peroxidation of muscle food as affected by NaCl, J. Agric. Food Chem., 1991, vol. 39, pp.1017–1021.
  24. Hernandez, P., Park, D., and Rhee, K.S., Chloride salt type/ionic strength, muscle site and refrigeration effects on antioxidant enzymes and lipid peroxidation in pork, Meat Sci., 2002, vol. 61, pp. 405–410.
  25. Lee, S.K., Mei, L., and Decker, E.A., Influence of sodium chloride on antioxidant enzyme activity and lipid peroxidation in frozen ground pork, Meat Sci., 1997, vol.46, p. 349–355.
  26. Ermakov, A.I., Arasimovich, V.V., Jarosh, N.P. Peruanskij, J.V., Lukovnikova, G.A., and Ikonnikova, M. I., Metody biohimicheskogo issledovanija rastenij(Methods of biochemical investigation of plants), Leningrad: Agropromizdat, 1987, pp. 41–45.
  27. World Health Organization; Reducing Salt Intake in Populations: Report of a WHO Forum and Technical Meeting, 5–7 October 2006, Paris, France; WHO: Geneva, Switzerland, 2007.
  28. Piul'skaja, V., Jekspress-metod jekstragirovanija zhira iz zhirovoj tkani (Express-Method of fat extraction from fat tissue), Miasnaia Industriia SSSR (Meat Industry of the USSR), 1958, no. 1, p. 9.
  29. Tarladgis, B.G., Watts, B.M. and Yonathan, M., Distillation method for the determination of malonaldehyde ?n rancid foods, J. of American Oil Chemistry Society, 1960, vol. 37 (1), pp. 44–48.
  30. Doronin, A.F., et al. Funkcional'nye pishhevye produkty. Vvedenie v tehnologii (Functional Food Products. Introduction into Technologies), Мoscow: DeLee print, 2009. 288 p.
  31. Lee, B.J., Hendricks, D.G., and Cornforth, D.P., A comparison of carnosine and ascorbic acid on color and lipid stability in a ground beef pattie model system, Meat Sci., 1999, vol. 51, pp. 245–253.
  32. Krzywicki, K., The determination of heme pigment in meat, Meat Sci., 1982, vol. 7, pp. 29–35.

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