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Title of article Theory and Practice of Prion Protein Analysis in Food Products
Authors

Prosyekov A., Kemerovo Institute of Food Science and Technology, The Russian Federation, Kemerovo, aprosekov@rambler.ru

Section: BIOTECHNOLOGY
Year 2014 Issue 2 DOI 641:577.112
Annotation The article presents the results of the research on methods of identification and quantitative determination of prion proteins in biological samples and multicomponent mixtures based on them. Analysis of nucleotide sequence of DNA encoding the PRNP gene of the prion protein, including phylogenetic and comparative analysis of nucleotide sequences of normal and pathogenic prion protein in cattle, was performed. Oligonucleotide primers for amplification of the PRNP gene of pathogenic prion protein were designed and synthesized. The high specificity of the developed test system was confirmed.
Keywords Prion, protein, encephalopathy, safety, quality, PCR, analysis
Artice information Received November 30, -0001
Accepted November 30, -0001
Available online November 30, -0001
Imprint article Prosyekov A. Theory and Practice of Prion Protein Analysis in Food Products / Prosyekov A. // Food and Raw Materials. - 2014. - no. 2. - P. 106-120
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