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Dyshlyuk L.S. , Kemerovo Institute of Food Science and Technology , bul`v. Stroitelei 47, Kemerovo, 650056 Russia ,

Novoselova M.V. , Kemerovo Institute of Food Science and Technology , bul`v. Stroitelei 47, Kemerovo, 650056 Russia

Rozalenok T.A. , Kemerovo Institute of Food Science and Technology , bul`v. Stroitelei 47, Kemerovo, 650056 Russia

Year 2013 Issue 2 DOI 663.15:620.3
Annotation Modern methods of chemical modification of enzymes conferring increased catalytic activity and stability to these molecules have been considered. The advantages of using magnetic nanoparticles for the production of stable immobilized enzyme preparations are presented. Chymotrypsin immobilization on Fe O nanoparticles modified with amino groups has been found to result in the incorporation of 88% of the enzyme into the solid phase. The change of the optimal pH and temperature ranges and an increase of stability of the immobilized chymotrypsin relatively to the respective characteristics of the native enzyme have been demonstrated.
Keywords catalytic activity, magnetic nanoparticles, chymotrypsin, glutaraldehyde method, immobilization
Artice information Received November 30, -0001
Accepted November 30, -0001
Available online November 30, -0001
Imprint article Dyshlyuk L.S. IMMOBILIZATION OF CHYMOTRYPSIN ON MAGNETIC Fe O NANOPARTICLES / Dyshlyuk L.S., Novoselova M.V., Rozalenok T.A. // Food and Raw Materials. - 2013. - №2. - С. 85-88
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  1. Volova, T.G., Biotekhnologiya (Biotechnology), Novosibirsk: Izdatel’stvo SO RAN, 1999.
  2. Biotekhnologiya (Biotechnology), Higgins, I., Best, D., and Jones, G., Eds., Moscow: Mir, 1988.
  3. Eggins, B., Khimicheskie i biologicheskie sensory (Chemical sensors and biosensors), Moscow: Tekhnosfera, 2005, no. 3, pp. 25-31.
  4. Gracheva, I.M., Tekhnologiya fermentnykh preparatov (Technology of enzyme preparations), Moscow: Agropromizdat, 1985.
  5. Antipova, L.V., Primenenie fermentov v pererabotke vtorichnogo molochnogo syr’ya (The use of enzymes in the processing of secondary raw materials of dairy origin), Moscow: AgroNIITEIMMP, 1992.
  6. Andreeva, I.N., Pantyukhin, A.V., and Sysuev, B.B. Immobilizatsiya fermentov i drugikh biologicheski aktivnykh soedinenii. Uchebnoe posobie (Immobilization of enzymes and other biologically active substances. Study guide), Pyatigorsk: Pyatigorskaya Gosudarstvennaya Farmatsevticheskaya Akademiya, 2001.
  7. Berezin, I.V. Biotekhnologiya. Uchebnoe posobie dlya vuzov. T. 7. Immobilizovannye fermenty (Biotechnology. Student training manual. Vol.7. Immobilized enzymes), Moscow: Vysshaya Shkola, 1987.
  8. Berezin, I.V., Klesov, A.A., and Shvyadas, V.K., Inzhenernaya enzimologiya (Engineering enzymology), Moscow: 1987.
  9. Dixon, M. and Webb, E., Fermenty. Tom 1,2 (Enzymes. Vols. 1, 2), Moscow: Mir, 1982.
  10. Berry, C., and Curtis, A., Functionalization of magnetic nanoparticles for applications in biomedicine, J. Phys. D. Appl. Phys., 2003, vol. 36, pp. R198-R206.
  11. Gu, H., Xu, K., and Xu, C., Biofunctional magnetic nanoparticles for protein separation and pathogen detection, J. of the American Chemical Society Chem. Commun., 2006, pp. 941-949.
  12. Massart, R., Preparation of aqueous magnetic liquids in alkaline and acidic media, IEEE Trans. Magn., 1981, vol. 17, no. 2, pp. 1247-1248.

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